primary antibodies against ang 2  (R&D Systems)

Journal: Arthritis Research & Therapy

Article Title: Double-antiangiogenic protein DAAP targeting vascular endothelial growth factor A and angiopoietins attenuates collagen-induced arthritis

doi: 10.1186/ar4265

Figure Lengend Snippet: Vascular endothelial growth factor A, angiopoietin 1 and angiopoietin 2 show different temporal patterns of expression during the course of collagen-induced arthritis . Collagen-induced arthritis (CIA) was induced, and joint extracts were isolated from normal DBA1 mice and from early (day 26), active (day 38) and chronic (day 52) CIA mice ( n = 4 for each group). (A) Representative sections of the ankle joints stained with hematoxylin and eosin (bars = 500 μm) from normal DBA1 and early, active and chronic CIA mice. (B) Sequential changes in the expression levels of angiopoietin 1 (Ang1) and Ang2 proteins in the joint tissue were determined by Western blot analysis. (C) The vascular endothelial growth factor A (VEGF-A) concentration in the joint tissue was determined by enzyme-linked immunosorbent assays. Values are mean ± SE. * P < 0.05 vs. normal.

Article Snippet: After blocking for one hour with 5% skimmed milk in a TBST buffer solution (10 mM Tris, 150 mM NaCl and 0.1% Tween 20), the membrane was incubated with primary antibodies against Ang-1 and Ang-2 (LifeSpan Biosciences, Seattle, WA, USA), followed by incubation with horseradish peroxidase-conjugated anti-rabbit immunoglobulin G (IgG) (Santa Cruz Biotechnology).

Techniques: Expressing, Isolation, Staining, Western Blot, Concentration Assay